A selective procedure for the field isolation of pathogenic Streptococcus spp. of rainbow trout (Salmo gairdneri).
نویسندگان
چکیده
A procedure established for the selective isolation of the species of Streptococcus responsible for rainbow trout streptococcosis in South Africa, consisted of the inoculation of samples into nutrient broth which had been supplemented with 100 micrograms/ml of nalidixic acid, 160 micrograms/ml of oxolinic acid or 200 micrograms/ml of sodium azide. After incubation, the sample was plated onto tetrazolium agar on which the rainbow trout pathogenic Streptococcus species grew as a red colony. The colonies were isolated from the tetrazolium agar and identified as rainbow trout pathogenic isolates by biochemical and serological tests. In the laboratory the selective procedure is capable of detecting about 2 bacteria per ml. This procedure was used in the field and biochemically identical Streptococcus species were found in the mud and a freshwater crab from the water source of a site with a history of streptococcosis.
منابع مشابه
The indirect fluorescent antibody technique for the rapid identification of streptococcosis of rainbow trout (Salmo gairdneri).
The indirect fluorescent antibody technique has been used successfully for the rapid serological identification of the Streptococcus sp. responsible for streptococcosis of rainbow trout. This technique has been used to identify the Streptococcus sp. in pure cultures and smears made from experimentally infected and diseased fish.
متن کاملPituitary activation by bacterial endotoxins in rainbow trout (Salmo gairdneri).
Endotoxins from Escherichia coli and Aeromonas salmonicida caused marked cortisol production in the rainbow trout (Salmo gairdneri).
متن کاملDistribution and Molecular identification of some causative agents of streptococcosis isolated from farmed rainbow trout (Oncorhynchus mykiss, Walbaum) in Iran
Over the past few years, the syndrome of streptococcosis has been associated with outbreaks in rainbow trout (Oncorhynchus mykiss, Walbaum) and caused significant economic losses in the aquaculture industry in Iran. The main purpose of this work was molecular identification of some causative agents of streptococcosis in rainbow trout. A total of 520 samples were collected from the head kidney o...
متن کاملDistribution and Molecular identification of some causative agents of streptococcosis isolated from farmed rainbow trout (Oncorhynchus mykiss, Walbaum) in Iran
Over the past few years, the syndrome of streptococcosis has been associated with outbreaks in rainbow trout (Oncorhynchus mykiss, Walbaum) and caused significant economic losses in the aquaculture industry in Iran. The main purpose of this work was molecular identification of some causative agents of streptococcosis in rainbow trout. A total of 520 samples were collected from the head kidney o...
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ورودعنوان ژورنال:
- The Onderstepoort journal of veterinary research
دوره 56 3 شماره
صفحات -
تاریخ انتشار 1989